EFFECT OF Mg ION ON HISTOCHEMICAL DEMONSTRATION OF ALKALINE PHOSPHATASE IN DECALCIFIED HARD TISSUE
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The effect of the presence of Mg<SUP>++</SUP> and Zn<SUP>++</SUP> upon the alkaline phosphatase activity of decalcified section was demonstrated in the following 4 groups histochemically.<BR>1) Group 1. (non-treated): A weak enzymatic activity was found in articular and hypertrophic cartilage cells in epiphysis.<BR>2) Group 2. (incubation medium containing Mg<SUP>++</SUP> or Zn<SUP>++</SUP> was used): A moderate enzymatic activity was found in articular and hypertrophic cartilage cells. The surface of primary bone trabeculae showed a strong stainability.<BR>3) Group 3. (pretreated for a short time): The enzymatic activity was moderate to high at the surface of epiphysial plate.<BR>4) Group 4. (pretreated for a long time): A strong enzymatic activity was found in the surface of epiphysial plate and of bone trabeculae. The surface of primary bone trabeculae was the strongest stainability.<BR>The difference of staining intensity between using Mg<SUP>++</SUP> and Zn<SUP>++</SUP> as metallic ion could not be demonstrated. Alkaline phosphatase activity of the decalcified hard tissue was increased by the presense of Mg<SUP>++</SUP> and Zn<SUP>++</SUP> histochemically.
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