QUANTITATIVE ENZYME HISTOCHEMISTRY OF NORMAL AND INJURED LIVERS : PART 2; ULTRAMICROCHEMICAL DETERMINATION OF NADP-DEPENDENT ISOCITRATE AND GLUCOSE-6-PHOSPHATE DEHYDROGENASE IN THE LOBULES OF NORMAL AND INJURED HUMAN LIVER

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Quantitative levels of glucose-6-phosphate dehydrogenase (G6PD) and isocitrate dehydrogenase (ICD) were determined in normal and injured human livers by the ultramicrochemical method of Lowry. G6PD in the normal hepatic lobule showed a central-portal gradient response with high activity in the central area. In the acute hepatitis and chronic hepatitis, liver G6PD activity increased and ICD activity decreased. In livers at the active stage of chronic hepatitis, G6PD activity increased and ICD activity decreased remarkably in the periportal area where piecemeal necrosis was observed. In liver cirrhosis some areas of hyperplastic nodule showed almost normal G6PD and ICD activity, while in internodular areas increased G6PD and decreased ICD activity were detected. In liver stroma in cirrhosis high G6PD activity and low ICD activity were detected.