Immunohistochemical Evaluation of the Effect of TZP-4238. Possible Androgen Receptor Antagonist in Rat Prostate.
スポンサーリンク
概要
- 論文の詳細を見る
Rat prostate was stained immunohistochemically using polyclonal androgen receptor antibody. A rabbit polyclonal antibody referred as NH27 was raised against human androgen receptor. Male SpragueDawley rats were divided into four experimental groups. Group 1 consisted of intact controls. In group 2, rats were sacrified two days after castration. In group 3, rats were administered subcutaneously 1mg/ animal of testosterone daily for three days after two days of castration. In group 4, rats were given 10mg/ kg of TZP-4238, an anti-androgen agent, orally by gavage once a day for 1 week. Frozen sections of the prostates were fixed in Zamboni's fixative. The sections were incubated overnight at 4°C with NH27. In the control rat prostate, positive staining for the antibody appeared in nuclei of the glandular epithelium. In addition, immunodetectable androgen receptor remarkably decreased 2 days after castration and returned to intact levels after testosterone-administration in the castrated rats. These findings are in agreement with results of previous work on the prostate. Furthermore, nuclear immunostaining of the androgen receptor remarkably decreased after the treatment with TZP-4238. The precise mechanism which produces atrophy of the prostatic glandular epithelium is not yet clear from the present study. We further speculated that TZP-4238 was considered to be a steroidal androgen receptor antagonist. It is concluded that immunohistochemical analysis using NH27 may be a very useful method for predication of the effects of antiandrogen treatment for rats.
- 日本組織細胞化学会の論文
日本組織細胞化学会 | 論文
- PII-27 中耳真珠腫に於ける5-FU軟膏局所投与の組織細胞化学的検討 : 角化細胞増殖因子(KGF)およびその受容体(KGFR)発現への影響(感覚器,ポスター,「出島」游學-形態通詞の未来展開-,第49回日本組織細胞化学会総会・学術集会)
- PII-16 ヒト乳突蜂巣の正常発育におけるアポトーシスの関与の検討(アポトーシス,ポスター,「出島」游學-形態通詞の未来展開-,第49回日本組織細胞化学会総会・学術集会)
- PII-13 ヒト大腸粘膜のリンパ管の同定、分布の検索 : IgG-gold silver法とエンビジョンキットを用いたDAB法の比較(免疫組織化学(III),ポスター,「出島」游學-形態通詞の未来展開-,第49回日本組織細胞化学会総会・学術集会)
- P-37 イムノブロット法は抗PYY, NPY, およびPP抗体の交差性検定によい手段である
- RNAiについての基礎と応用(E.核酸・タンパクの修飾と可視化および硬組織の組織化学)