LIGHT AND ELECTRON MICROSCOPIC IMMUNOHISTO-CHEMICAL LOCALIZATION OF PEPTIDYLGLYCINE ALPHA- AMIDATING MONOOXYGENASE (PAM) IN THE RAT ENDOCRINE ORGANS

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The distribution of PAM was studied immunohistochemically at light and electron microscopic levels in the endocrine organs of mature male rats. PAM was found in the anterior pituitary gland, thyroid C cells, the gastrointestinal tract, the pancreas, and the adrenal medulla. For light microscopic immunohistochemistry, fixative B (pH 4 Bouins) and paraffin embedding were satisfactory with SDS treatment preceding immunohistochemical staining. But PAM in the thyroid C cells could be detected only by frozen sections after Zamboni fixation. By the immunoelectron microscopy pre-embedding method, PAM was localized in secretory granules suggesting the site of a-amidation in secretory granules. Our studies emphasized the role of the secretory granules in posttranslational processing not only in proteolytic digestion but also in α-amidation.