分離精製 : 微量・大量生産

概要

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As biologically active substances, various kinds of proteins have been discovered and utilized for pharmaceuticals. It is very difficult to obtain enough amount of the proteins from natural source. Therefore, cell culture and DNA recombinant technology has been developed and made it possible to supply large amount of the proteins. The purification of proteins mainly consists of three steps, initial treatment, concentration or fractionation, and column chromatography. New and efficient separating materials for affinity chromatography and HPLC have considerably improved the isolation of proteins on the small and large scales. The amount of impurities, the most important being endotoxin, nucleic acids, and host cell proteins, are usually in the picogram range, and thus at the limit of sensitivity of the currently available methods.
社団法人有機合成化学協会の論文