DNAの化学合成における最近の進歩

概要

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Recent developments in the field of oligodeoxyribonucleotide synthesis were described. Novel coupling systems such as new condensing reagents for modified phosphotriester method, phosphite coupling and its modified method, and activated phosphotriester method were discussed. On the other hand, newly developed protective groups of guanine and adenine moieties, partial deprotection systems for trityl groups, and widely developed polymer support synthesis were also described. The efficiency of these methods was obvious in terms of yields and time, so one can construct a defined oligodeoxyribonucleotide more easily and rapidly. And further investigation of the deprotection and purification systems by using high performance liquid chromatography let us obtain the pure DNA fragments.
社団法人有機合成化学協会の論文