海洋および湖沼堆積物中の有機酸の定量
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By examining methods for extraction, identification and determination of organic acids in marine and lake sediments the following was established as a recommended procedure. Add 100ml of extractant (0.5N H2SO4-80% EtOH) to 20g of the sediment sample and stir vigorously for 48 hours at room temperature. Then separate the supernatant fraction containing organic acids by centrifugation. Extract the residual organic acids repeatedly from the precipitates with the above treatments for three times, and collect all the supernatant fractions obtained. Neutralize the extract (collected supernatant fractions) with NaOH, and condense it to dryness by evaporation at 100°C, and then redissolve this dried extract by adding a small amount of H2SO4. Extract again the organic acids from this acidified solution (pH 1-2) with ether using NEISHs continuous extraction method4). The organic acids contained in this ether solution can be identified and determined by use of MUELLERs chromatographic method5). Identification and determination of organic acids in sediment samples from Maizuru Bay and Lake Biwa were carried out by use of the above procedure and the following results were obtained. 1. Sediment samples contained acetic, formic, lactic, propionic, butyric, and an unidentified organic acid, regardless of their origin and depth. Among these acetic acid was found in the highest concentration in general, and the unidentified acid was detected in the lowest concentration. 2. The concentration of organic acids in the sediments decreased generally with the depth from the surface of sediments.
- 公益社団法人 日本水産学会の論文
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