病原性水生糸状菌の生理生態に関する研究-1-

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The experiments and observations concerning the life cycle and pH suitable for growth of the pathogenetic Saprolegnia spp. isolated from some fungi-diseased fishes, eels and eggs of fish, showed the following results. (1)Almost all the fungi were identical with Saprolegnia parasitica COKER. Some of the rest seemed to be other strain belonging to the same species, but could not be correctly identified. A few were believed to be the other strain that have rather stronger resisting power against malachite green. (Table 1) (2) In simple-pepton-medium, the micellium continued only to grow, whereas in the dist. water they formed zoosporangia. Gemma were formed in low temperature (below 15°C) or after a long culture-period. Considering that gemma formed numerous zoospores in them, just as zoosporangium did, it seemed that there might be no essential difference between zoosporangia and gemma. (3) The growth ratio of the mycellium measured in volume, was shown as a sigmoidcurve that had rather long straight part. (Fig. 1) (4) The same strain had almost the same top-point in optimum pH, near the neutral point, for growing. But some of them seemed to show the after-effect of their native ponds. (Fig 2) A pH value of the culture-medium gradually approached to the optimum value in the course of culture. (Fig 3) Correlation between growth and pH, in agar-plate-culture was quite different from that in solution culture.
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