A voltage clamp method with internal perfusion for quasi-intact axon of the crayfish

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概要

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• A voltage-clamp system with internal perfusion for quasi-intact crayfish giant axons was developed using a partition method where three pools were formed by two partitions. The membrane potential was extracellularly recorded across the one side partition whose resistance was highly increased using a water-absorbing material, Sephadex G25 mixed with vaseline and liquid paraffin so that no short circuiting correction became necessary. Axon membranes were voltage-clamped at the center by feeding current through the other side pool. For the internal perfusion with the axoplasm left intact, the membranes of the lateral pools were perforated with a 0.1% of saponin-treatment for 1.5-2min, through which the axon was intracellularly“perfused”with a reasonable diffusion rate. This system was well bearable to long-term experiments under both extracellularly and intracellularly controlled conditions of the cell.

• 日本学士院の論文

著者

• HIRONAKA Tetsuji

  Department of Pharmacology, Teikyo University School of Medicine

• IKARI Yoshio

  Department of Pharmacology, Teikyo University School of Medicine

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• A method for measuring resting and action potentials with external electrodes in crayfish giant axons
• A voltage clamp method with internal perfusion for quasi-intact axon of the crayfish

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