A RAPID AND SIMPLIFIED DETERMINATION OF TYROSINE HYDROXYLATION

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概要

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• The present paper reported a modification of the assay method for evaluating tyrosine hydroxylation described by Nagatsu, Levitt and Udenfriend. The procedure excluded adsorption step of <SUP>3</SUP>H-tyrosine and <SUP>3</SUP>H-dopa to Dowex or charcoal adsorbent for removal of <SUP>3</SUP>H-water, and following elution step from the adsorbent. The radioactivity of <SUP>3</SUP>H-tyrosine remaining in the reaction mixture or <SUP>3</SUP>H-water converted from <SUP>3</SUP>H-tyrosine was easily measured with this procedure without any skilful technique. It is needless to say that this method will give more satisfactory results if aliquot of the mixture is accurately transf erect in quantity to a scintillation vial.

著者

• HIDAKA TOSHIHIRO

  Department of Biochemistry, Kurume University School of Medicine

関連論文

• A RAPID AND SIMPLIFIED DETERMINATION OF TYROSINE HYDROXYLATION

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