Establishment of a new human gallbladder carcinoma cell line (KMG-A) from an alpha-fetoprotein-producing gallbladder carcinoma transplanted into nude mice.

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An alpha-fetoprotein (AFP)-producing human gallbladder carcinoma with direct invasion to the liver was transplanted into BALB/c-nu/nu nude mice and a new human gallbladder carcinoma cell line (KMG-A) has been established from the transplanted tumor. The patient's serum levels of AFP and carcinoembryonic antigen (CEA) were elevated at 1, 040 ng/ml and 22.1 ng/ml, respectively after the operation. The tumor transplanted into nude mice was a poorly differentiated adenocarcinoma showing both solid and papillo-tubular structures. Serum levels of AFP, CEA, carbohydrate antigen 19-9 (CA 19-9), β<SUB>2</SUB>-microglobulin (BMG), and ferritin (FER), were elevated in the nude mice with tumor transplants. The KMG-A cell line has been maintained for 2 years through 80 passages. Morphologic features of KMG-A cells contained varying-shaped and-sized cells, including some multinuclear giant cells. KMG-A cells had one or more large round to oval nuclei with prominent nucleoli. The doubling time of cells grown in a serum-containing medium was about 28.5 hours and KMG-A cells were able to proliferate in a serum-free medium. Functionally, KMG-A cells produced CA 19-9, BMG, and FER, but AFP and CEA were not detectable in the supernatant of KMG-A cells. KMG-A cells were identified as containing AFP, CEA, CA 19-9, and albumin (ALB) by immuno-histochemical techniques. The tumorigenicity of KMG-A cells was not identified by the subcutaneous inoculation of the cells into nude mice 4 months after inoculation, but histological examination of the subcutaneous tissue where the KMG-A cells were inoculated revealed small foci of poorly differentiated adenocarcinoma.
久留米大学医学部 The Kurume Medical Journal 編集部の論文

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