放射性鉄靜注に依る鉄代謝の研究 : 特にNon-hemin鉄代謝を中心として
スポンサーリンク
概要
- 論文の詳細を見る
(I) Experimental method.1) Viviperfusion: rabbits and dogs were viviperfused for 30-60 minutes.2) Digestion: samples were wet ashed in Kjeldahls flasks.3) Electroplating: Vosburghs method.4) Fractionation of non-hemin iron: Yoneyamas method.5) Extraction of non-hemin iron: Brueckmanns method.6) Determination of serum iron: Burchs method.7) Determination of radioactivity: by Geiger Müller counter.8) Histological iron-staining: by the turnbull-blue reaction method.(II) Conclusions1) For the studies on the physiological iron-metabolism, amount of intravenously injected iron should be within the limit of the iron-binding capacity of serum.2) Intravenously administered radioiron is taken most intensively by the bono-marrow and next by the liver; in the bone-marrow it is mainly used for the synthesis of hemoglobin, while in the liver it is deposited as non-hemin iron.3) When animals are bled the deposited radioiron is mobilized from liver, bone-marrow and other organs for the synthesis of hemoglobin. In such condition hemosiderin seems to be utilizated for the synthesis of hemoglobin.4) In the hemorrhagic iron deficient anemia the uptake of radioiron by the bone-marrow is increased, but the uptake by the liver is decreased in compaired with normal animals. On the other hand, in the acute inflammation and X-ray irradiated anemia it was decreased in the bone-marrow.5) The iron deposition following intravenous injection of red cells ragged with radioiron is mainly seen in the spleen. In such experiment radioiron is found in the ferritin, therefore iron of ferritin seems to be drived from hemoglobin iron too.
- 社団法人 日本内科学会の論文