血液凝固にかんする研究-2-
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Presented in this paper are the results of some experimental studies on the action of serum clotting factors as to the formation of active prothrombin-converting-principle or blood-thromboplastin, and on the assay of Factor VII activity in serum.These investigations arose from the study of two siblings with congenital bleeding tendency of Factor VII deficiency type, as judged by prothrombin time tests. During the study of the patients it was possible to observe the effects on blood-thromboplastin formation of mixtures of some pathological sera.1) It was suggested that the Factor VII deficient serum has no or little effect on the result of thromboplastin generation test of Biggs and Douglas. It appeared, on the other hand, that the concentration of Factor VII in the reagent has a great effect on the result of the test in which brain extract is used as a reagent instead of platelet suspension.2) The sera of patients with severe liver injury, and patients treated with Phenylindandione are deficient in Christmas factor as well as Factor VII. From the results of mixture-experiments, the possibility of the existence of third serum clotting factor was inferred, but if this factor is identical with Factor X of Koller has not yet been made. This factor is deficient in patients suffering from severe liver injury, and in patients treated with phenylindandione.3) It is advisable to use acid-treated-serum as for Factor VII-poor-serum, since the asbestos-filtered-serum lacks much quantity of Christmas factor.4) A method of assay of Factor VII activity in serum, based on the thromboplastin generation test in the presence of brain extract, was described.The Factor VII activity in the serum of 50 healthy adults was found to vary between 64 to 114 percent.Furthermore, serum samples from 21 cases with coagulation defects were examined. The results obtained by the method correlated well with SPCA sctivity obtained by the method of de Vries et al..
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