胃液蛋白のPolarograph的研究-1-
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概要
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An analysis of various interfering conditions in polarogram formation was conducted as a preliminary study of the polarographic characteristics of cancerous gastric juice and the following results were obtained.1) Changes in polarographic waves induced by pH adjustment.When the polarogram of gastric juice was observed using Co++ and Co+++ as electrolytes, it was noted that in anacidic specimen, marked variations in polarogram formation were present.Likewise, when specimens were left standing at room temperature, the variations were also distinct. As regards the influence induced by pH adjustment, it was noted that variations in polarogram decreased in the descending order: cancer>anacidic>acidic with the cancerous polarogram showing the highest degree of lability.2) On the influence of degree of viscosity of gastric juice.There was no correlation between the viscosity of gastric juice and its polarographic wave height. In other words, the influence was negligible and can be disregarded.3) On the influence of saliva.When a large amount of saliva is added to the gastric aspirate, the polarogram in Co++ shows a lowering in W1, while the reduction voltage in W2 shows a tendency to shift to the negative side which is due to the polarographic activity of the saliva itself. However, the said influence may readily be prevented by avoiding saliva contamination when gastric juice is aspirated and by using Co+++ electrolyte.4) On the influence of blood and bile.Both blood and bile present a double wave similar to that of gastric juice. Hence, when both or either are added to gastric juice the resulting wave height changed in polarograms appears as an average total of the mixture. However, since the samples are highly colored in this case, the influence of blood and bile may also disregarded. It is likewise advisable to use Co+++ electrolyte.Summarizing the above, it may be concluded that in the studies of the characteristics of polarographic wave heights in cancerous gastric juice, interference by pH adjustment, juice viscosity, and contamination by saliva, blood and bile are negligible and may be disregarded.
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