血液アミノ酸に關する研究 : 第2報 Paper Chromatographyに依る正常人血液各分劃アミノ酸檢索
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Free and constituent amino acids in every fraction of normal human blood, separated as purely and intactly as possible, in accordance with the method previously reported by author, was detected semi-quantitatively by two-dimensional paper chromatography over and over again. The results of which are outlined as follows:1) The most outstanding fact is that free taurine exists in great quantity in the fraction of platelets as compared with other fractions. This taurine seems to exist only in free state.2) The spot of asparagine in hydrolysate is clearly noticeable only in the fraction of platelets but hardly any at all in every fraction in free state.3) In the hydrolysate, “under-alanine”, histidine, proline, arginine, threonine, tyrosine and phenylalanine are either slightly observed in the platelets fraction, or not observed at all, whereas they are remarkably observed in other fractions, particularly in white cells fraction.4) Generally speaking, free amino acids tend to concentrate on blood cells fractions rather than plasma, particularly on platelets and white cells fractions. On the other hand, the constituent amino acids spots increase quite noticeably on both variety and intensity than that in free amino acids spots, without concentration on the blood cells fractions. But in anyway no remarkable difference in patterns is found between the fractions.5) Essential amino acids, in free state, are all thin and what are found in each fraction are spots of valine, leucine, isoleucine, methionine and phenylalanine, but all of essential amino acids are observed in the hydrolysates.6) S-compounds like taurine, cystine, cysteic acid, tetramethylcystine?, glutathione, etc. concentrate on blood cells fractions in free state, particularly, on platelets and white cells fractions but hardly any are observed as a constituent.7) Glycine is only just found, in free state, in fractions of platelets and white cells, but as a constituent a quantity of it is observed in all fractions.8) In general, the intensity of carbonic amino acids spots are much greater than that of basic amino acids spots throughout all samples.9) Several unidentified substances are found present in various quantity in some fractions.
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