THE BASIC IMMUNOLOGICAL STUDIES ON EXPERIMENTAL SCHISTOSOMIASIS JAPONICA

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The rabbits were infected with about 500 cercariae of Schistosoma japonica. The localization, specificity, and change of the antigen antibody reaction in adult worm, ovum, and miracidium and granuloma with ova in the organs after infection of cercariae were observed by the direct and indirect fluorescent antibody technique. At the same time, rise and fall of the antibody titer was serologically observed in the precipitin test and the tannic acid hemagglutination test. The liver function tests were performed to study of destruction of the liver. Furthermore, after infection, the transformation of the rabbit serum fraction by the immunoelectrophoresis was studied. The results of the above experiments were as follow.1) In the precipitin test and the tannic acid hemagglutination test using adult worm as antigen, antibody titer rose in 6-8 weeks after infection and it continued until 20 weeks.2) It was observed that the serum protein fraction and the transaminase changed remarkably. Albumin fell and beta-, gamma-globulin increased in 8 weeks after infection. Gamma-globulin tended to increase more in 20 weeks after infection. Both s-GOT and s-GPT increased remarkably in 4 weeks after infection and decreased remarkably in 8 weeks, it increased and decreased until 20 weeks at the line of the normal rate.3) Specific strong precipitate line in beta-globulin region was observed in those 2, 4, 6, 8, 10, 12, 14, and 20 weeks after infection by immunoelectrophoresis.4) The direct fluorescent antibody technique was applied to cercaria, adult worm and miracidium and the fluorescein-labelled infected rabbit gamma-globulin with Schistosoma japonica. The specific fluorescence was seen in each. It was hard to deny that common antigen existed in each. The fluorescent reaction of cercaria, adult worm, and miracidium of Schistosoma japonica in each period (6-, 8-, 12-, and 20 weeks after infection) was not found to differ. Meanwhile, slight fluorescence was also observed in the control which was normal rabbit gamma-globulin.5) In the indirect technique, fluorescent reaction of ccercaria, adult worm, and miracidium was observed in untreated rabbit and infected rabbit from 2 weeks to 20 weeks after infection.In cercaria, slight fluorescent reaction was observed from 2 weeks to 20 weeks after infection. (F. A. dilution 4×-16× dilutions, serum dilution 10×-100× dilutions)In adult worm, no fluorescence was seen in them until 4 weeks after infection but seen slightly from 8 weeks to 20 weeks. (F. A. dilution 8×-32×, serum dilution 10×-100×)In miracidium, no fluorescence was seen in them until 6 weeks after infection but seen between 8 weeks and 20 weeks. (F. A. dilution 4×-16×, serum dilution 10×-l00×).6) Markedly different findings were seen in the fluorescent reaction to labelled gamma-globulin in the infected rabbit organs.Bright apple-green like specific fluorescence to labelled gamma-globulin 6, 8, 12, and 20 weeks after infection was observed in the ovum of the liver and intestine in the direct technique. However, very slight fluorescence was seen in the granuloma.Meanwhile, in the indirect technique, bright apple-green fluorescence was seen in the ovum, the granuloma around the ovum of the liver, intestine, and the red pulp of the spleen. However, this reaction was partially seen in the control.7) No fluorescence was seen in the normal organs.
久留米大学医学部 The Kurume Medical Journal 編集部の論文

THE BASIC IMMUNOLOGICAL STUDIES ON EXPERIMENTAL SCHISTOSOMIASIS JAPONICA

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