エタノール局所注入後の肝組織壊死と線維化に関する検討

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Absolute ethanol has been widely proposed for several years as an agent for therapeutic injection against small hepatocellular carcinomas. We measured necrotic areas in normal rat livers caused by injections of varied concentrations of ethanol (25%, 50%, 75%, 99.5%) after a period of 72hr. Necrotic areas increased significantly in relation to increasing ethanol concentrations (p<0.01). We have reported that desmin existed in cytoplasma of Ito cells and that, conversely, Ito cells were easily found by desmin staining of damaged livers. Fibrosis in hepatic lobules was suggested to depend upon Ito cells. We observed Ito cells and hepatic fibrosis after absolute ethanol injections by using desmin staining. After 24hr, the number of Ito cells slightly increased around necrotic areas and polymorphonuclear leukocytes infiltrated into necrotic areas. After 48hr, Ito cells were observed around necrotic areas, formed into layers, and their cytoplasmic processes were elongated. However, fibrosis was slight. After 72hr, coagulation necrosis was apparently observed and fibrous bands were evident. Ito cells increased in number around necrotic areas, and their cytoplasmic processes elongated, and they attached themselves to that of neighboring cells. Ito cells decreased with the progress of fibrosis thereafter. These findings suggest that Ito cells show a close relationship to hepatic fibrosis.
社団法人日本肝臓学会の論文