Morphological analysis for gene expression of .GAMMA.-glutamyltranspeptidase in rat liver during carcinogenesis induced by diethylnitrosamine.

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We investigated the expression of γ-glutamyltranspeptidase (γ-GTP) in rat liver during carcinogenesis induced by diethylnitrosamine (DEN), using both immunohistochemical and in situ hybridization techniques. At 1 month after DEN injection, γ-GTP immuno-positive cells (parenchymal cells) were scattered in the zone I and zone II of liver lobules. And at 2 months, γ-GTP immuno-positive enzyme altered foci (EAF) were observed predominantly in the zone I and zone II. At 5 months, γ-GTP immuno-positive hyperplastic nodules (HNs), which compressed surrounding tissue, were also seen in liver lobules. γ-GTP immuno-positive structures were observed at cell membrane and bile canaliculi. Using <I>in situ</I> hybridization, we detected assemblies of silver grains which indicated mRNA, in both the scattered hepatocytes in the early stage of hepatocarcinogenesis and the cells in hyperplastic foci. These results suggested that the hepatocytes expressing γ-GTP in zone I and zone II might be concerned with the genesis of EAF and HNs.