The MTT assay for evaluation the cell viability and the cell injury of cultured hepatocytes.

概要

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We applied the MTT (3-(4, 5-dimethylthiazo1-2-y1)-2, 5-diphenyltetrazolium bromide) assay to the primary culture of rat hepatocytes, and observed good correlation between the cell number of viable hepatocytes and the optical density of the MTT formazan. This result shows that the viability of cultured hepatocytes can be evaluated by the MTT assay. Using this assay, we studied the effect of chenodeoxycholic acid (CDCA) on the viability of cultured hepatocytes in varios concentrations and incubation times. The viability of cultured hepatocytes declined with increased concentration and incubation time of CDCA. In addition, we found that nicardipine, a Ca2+ channel blocker, prevented hepatocytes from the CDCA-induced cell injury. This result suggests that Ca2+ plays an important role in the CDCA-induced cell injury.