C型肝炎ウイルスにおける表面抗原検出系の確立 : 高変異領域の関与について
スポンサーリンク
概要
- 論文の詳細を見る
E2/NS1 region, putative surface antigen of hepatitis C virus (HCV), contains two hypervariable regions (HVRs). To get insight of the clinical significance of HVRs, detecting system for E2/NS1 protein was established using 5 kinds of polyclonal antibodies against recombinant proteins of the regions expressed in E. coli.<BR>As low as 10 ng of the recombinant proteins were specifically detected in this system. Crossreaction studies of the 5 antibodies against the proteins suggested that heterogeneity of amino acid sequences in HVRs is closely correlative with affinity of the antibodies. A protein corresponding to size of 44 kDa was specifically observed in a liver sample from a patient with chronic active hepatitis C using this system. Also, reactivity of the 44 kDa protein against the antibodies was different among these 5 antibodies.<BR>Thus, these results indicate that HVRs may have an important role on immune system against HCV.
- 日本肝臓学会の論文
日本肝臓学会 | 論文
- 低エコーを呈し, 経過観察中に増大傾向をみた肝血管筋脂肪腫の1例
- 免疫グロブリン製剤によるA型肝炎の感染予防に関する研究
- 無症候性HBs抗原保有者の生化学的ならびに免疫学的背景に関する研究
- 医療従事者におけるB型肝炎ウイルス感染の疫学的研究
- 劇症肝炎の臨床像に関する研究