ブタ肝よりのGlutamic Oxaloacetic Transaminaseの精製と免疫学的研究

概要

論文の詳細を見る
GOT isozymes of pig liver have been purified each other, analysed by immunodiffusion and immunoelectrophoresis in agar gel, and further their hapatic cellular sites have been investigated by indirect fluorescent antibody method; supernatant GOT (s-GOT) and mitochondrial GOT (m-GOT) are quit different enzyme proteins immunologically, and the former consists of four subconponents migrating to the anode, while the later includes two subcomponents moving to the cathode and one to the anode. The specific fluorescence of s-GOT is seen in very small granules within hepatic cells, of which some positively stained cells are occuring singly or in groups throughout the lobules. Most of positively stained cells are found in the peripheral zone of the lobules. m-GOT positive cells are rather diffusely distributed throughout the lobules, and its distribution pattern is different from that of s-GOT. Cells other than hepatocytes, Kupper cells and endothelial cells in the sinusoid are not stained for s-GOT or m-GOT.
社団法人日本肝臓学会の論文