Labeling of Vestibular Afferent and Efferent Nerves from Selected End-Organs in Chinchillas.
スポンサーリンク
概要
- 論文の詳細を見る
A new method was developed for specific labeling of vestibular afferent and efferent neurons from selected end-organs. Biocytin solution was applied to the inner ear after the surface of the sensory neuroepithelium of an end-organ had been scratched. After 24 hours, the animal was perfused with a fixative and its brainstem was removed. The brainstem and vestibular nerves were serially sectioned with a freezing microtome, and 40 μm slices were processed with ABC solution. Diaminobenzidine (DAB) was used as the chromagen. Sections were mounted on slides and coverslipped for light microscopic observation. The distribution of primary afferent fibers from the horizontal semicircular canal (HSC) and the saccule (SAC) in the brainstem was as previously reported (Lee WS, 1992). The selectivity of efferent labeling was verified by the distribution of afferent fibers labeled simultaneously. A group of vestibular efferent neurons was found in the dorsal area of the brainstem ventromedial to the medial vestibular nucleus and lateral to the facial genu. Another group of efferents was situated ventrally in the reticular formation. Dorsal group efferent neurons innervating HSC were located more caudally than SAC efferents and were primarily on the contralateral side. In the reticular formation, SAC efferents were found more on the contralateral side, while HSC efferents were found bilaterally. This method may contribute to the detailed anatomical study of the vestibular efferent system.
- 一般社団法人 日本めまい平衡医学会の論文
一般社団法人 日本めまい平衡医学会 | 論文
- :especially on the potentiation effect of Scopolamine
- Effect of alcohol on compensation after unilateral labyrinthine loss.
- タイトル無し
- Complex Congenital Nystagmus; A Case Report.
- Sensation of Dizziness. Correlations of Direction of Rotation and Direction of Nystagmus.:Correlations of Direction of Rotation and Direction of Nystagmus