Investigation of the optimal condition for the induction of Cytotoxic T Lymphocytes(CTL) and application of CTL to gynecologic malignancies.
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概要
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We investigated optimal conditions for induction of cytotoxic T lymphocytes (CTL) by in vitro sensitization (IVS). Each peripheral mononuclear cells from 8 patients with gynecologic malignancies were incubated with MMC-treated cancer cells for 18 days with rIL 2. The degree of both antitumor activity and proliferation of CTL was affected by the IL-2 concentration and the ratio of number of MMC-treated cancer cells to that of lymphocytes used for culture. Incubation with 800 JRU/ml of rIL 2 produced maximal CTL induction. And at the ratio of 1:10, maximal CTL induction was attained. Thus CTL was optimally induced by mixed cultivation of the lymphocytes and cancer cells at the 10:1 with rIL 2 added at the concentration of 800 JRU/ml. The 4 patients with gynecologic malignancies were treated with CTL and rIL 2. The CTL therapy was effective for peritnitis carcinomatosa in these patients, but no objective regressions of cancer were observed.