Characterization and response to human interleukin 3 and 4 of pokeweed mitogen-activated peripheral blood mononuclear cells.
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概要
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Responses of peripheral blood mononuclear cells (PBMC) that had been stimulated by various mitogens to human recombinant cytokines were examined. PBMC that had been stimulated by T-cell mitogen, PHA and Con A, responded to recombinant IL-2 remarkably. However, they did not respond to recombiant IL-3 and IL-4. On the other hand, PBMC that had been activated by T and B cell mitogen, pokeweed mitogen (PWM) and Staphylococcus aureus cowan I (SAC-1), responded to IL-3 and IL-4 significantly as well as to IL-2. Especially, PWM-stimulated mononuclear cells (PWM-blasts) responded well to human recombinant IL-3 and IL-4, and they showed marked DNA synthesis. PWM-blasts did not respond to IL-1, IFNγ, GM-CSF, G-CSF, M-CSF, IL-5 and IL-6. However, they showed significant response to TNF and lymphotoxin, as well as to IL-2, IL-3 and IL-4. PBMC that had been preincubated for 4 or 5 days incorporated 3H-thymidine in dose-dependent way, when they were cultured in the presence of rIL-3 or rIL-4. Responses of PWM-blasts to IL-3 were inhibited specifically, when anti-IL-3 monoclonal antiboby was added to the culture. IL-2 dependent cell line, CTLL-2, did not respond to human rIL-3 nor rIL-4, suggesting that combination of CTLL-2 assay and PWM-blast method enables us to discriminate between human IL-2 and IL-3/IL-4 activity in simple and convenient way. CD2+, CD4-, CD8- cells were shown to play an important role in IL-3 responsive cells of human peripheral blood.
- 日本臨床免疫学会の論文
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関連論文
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- Characterization and response to human interleukin 3 and 4 of pokeweed mitogen-activated peripheral blood mononuclear cells.