In vitro immunoglobulin synthesis of peripheral blood lymphocytes from patients with SLE and normal subjects

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Enzyme immunoassay (EIA) was applied for measurement of immunoglobulins (IgA, IgG, and IgM) released from the cultured lymphocytes of normal subjects and patients with SLE.The peripheral blood lymphocytes (1×106 cells/ml) were cultured in RPMI 1640 medium supplemented with 20% fetal calf serum at 37°C for 7days in 5% CO2 incubator.IgA, G, and M levels in supernatant of the culture of normal subject (N=20) were 284±294 (Mean±SD), 303±203, and 59±68, respectively. In patients with active SLE (N=13), IgA and IgG levels in supernatant were significantly higher than those in normal subjects (IgA 699±830, IgG 1777±1286), however, in patients with well controled disease (N=7) Igs levels were similar to those of normal subjects.When the lymphocytes of normal subjects were stimulated with PWM, IgM and IgG levels markedly increased with unchanged IgA levels. In patients with active disease, all Igs levels decreased rather than those of non-stimulated cultures.When the lymphocytes were stimulated with Con A and PHA, Igs production were markedly suppressed in both two groups. However the degree of suppression by both Con A and PHA in patients with active disease were statistically weaker than those in normal subject.The results indicated 1) EIA can detect the small amount of Igs released from cultured lymphocytes, 2) in vitro Igs production and the effects of PWM in patients with SLE may reflect some T-cell defects and disease activity, and 3) this simple method may be available for evaluation of immunological disorders.
日本臨床免疫学会の論文

In vitro immunoglobulin synthesis of peripheral blood lymphocytes from patients with SLE and normal subjects

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