Enhancement of the binding of O-ethyl O-p-nitrophenyl phenylphosphonate (EPNoxon) to microsomal carboxylesterase by NAD in vitro.
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概要
- 論文の詳細を見る
Inhibition of rat liver microsomal carboxylesterase(CEase) by O-ethyl O-p-nitrophenyl phenylphosphonothioate (EPN) and binding of EPN oxygen analog to microsomal CEase were enhanced by addition of NAD or NADP. This was more prominent in addition of NAD than NADP. No potentiation of anti-CEase action of EPN by NAD was seen when pure esterase (E.C. 3.1.1.1) instead of liver microsomes was used as an enzyme source. This effect of NAD in microsomal CEase was significantly decreased when N-ethylmaleimide or p-chloromercuribenzoic acid was added. From these findings, it is strongly suggested that NAD-mediated potentiation of the anti-CEase action of EPN might be attributed to the increase in formation of NADH from NAD by microsomal dehydrogenase(s) containing a sulfhydryl group, leading to a subsequent increase in formation of the EPN oxygen analog from EPN, and in turn, CEase inhibition was enhanced.
- 公益社団法人 日本薬理学会の論文
著者
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Igarashi Takashi
Laboratory Of Biochemical Pharmacology And Biotoxicology Faculty Of Pharmaceutical Sciences Chiba Un
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Ueno Koichi
Laboratory Of Biochemical Pharmacology And Biotoxicology Department Of Drug Evaluation And Toxicolog
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Satoh Tetsuo
Department Of Anesthesiology National Defense Medical College
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Hosokawa Masakiyo
Laboratory Of Biochemical Pharmacology And Biotoxicology Faculty Of Pharmaceutical Sciences Chiba Un
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UENO Koichi
Laboratory of Biochemical Pharmacology and Biotoxicology, Department of Drug Evaluation and Toxicological Sciences, Faculty of Pharmaceutical Scierces, Chiba University
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SUGIYAMA Seiyu
Laboratory of Biochemical Pharmacology and Biotoxicology, Department of Drug Evaluation and Toxicological Sciences, Faculty of Pharmaceutical Scierces, Chiba University
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KITAGAWA Haruo
Laboratory of Biochemical Pharmacology and Biotoxicology, Department of Drug Evaluation and Toxicological Sciences, Faculty of Pharmaceutical Scierces, Chiba University
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KITAGAWA Haruo
Laboratory of Biochemical Pharmacology and Biotoxicology
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HOSOKAWA Masakiyo
Laboratory of Biochemical Pharmacology and Biotoxicology, Department of Drug Evaluation and Toxicological Sciences, Faculty of Pharmaceutical Scierces, Chiba University
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UENO Koichi
Laboratory of Biochemical Pharmacology and Biotoxicology
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IGARASHI Takashi
Laboratory of Biochemical Pharmacology and Biotoxicology, Department of Drug Evaluation and Toxicological Sciences, Faculty of Pharmaceutical Scierces, Chiba University
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