Effect of progesterone and its 17.ALPHA.-hydroxyl derivative on human erythrocyte membrane.

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概要

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• Effect of progesterone and 17α-hydroxyprogesterone on human erythrocyte membrane was investigated by three methods. 1) Prompt hemolysis test: Progesterone progressively reduced the membrane fragility at the concentration of 1.25×10<SUP>-5</SUP>-2.0×10<SUP>-4</SUP> M, but 17α-hydroxyprogesterone had almost no effect. 2) Continuous MCV analysis: When erythrocytes were transferred into 0.9 (w/v) % NaCl solution without progesterone after incubation with 0.5 M d-glucose for 2 hr at 37°C, erythrocytes markedly expanded up to about 140 μm<SUP>3</SUP> and subsequently returned to nearly the original volume (about 95 μm<SUP>3</SUP>) within 30-40 sec. Progesterone reduced the reversibility of expanded erythrocytes at the concentration of 1.25×10<SUP>-5</SUP>-1.0×10<SUP>-4</SUP> M. 17α-Hydroxyprogesterone had little effect on the reversibility. 3) Scanning electron microscopy: After incubation in isotonic buffered saline solution without progesterone for 12 hr at 37°C, most of the erythrocytes were transformed from discocytes to echinocytes and spheroechinocytes (echinocytes+sphero-echinocytes, >80%). Progesterone (5.0×10<SUP>-5</SUP> and 1.O×10<SUP>-4</SUP> M) inhibited this transformation, so that a greater number of erythrocytes remained as discocytes than in the control. Moreover, at higher concentration of progesterone, stomatocytes emerged prominently (1.0×10<SUP>-4</SUP> M progesterone), followed by the increase in spherocytes (2.0×10<SUP>-4</SUP> M). 17α-Hydroxyprogesterone, however, merely prevented the echinocytic progress from I to II or III at the concentration of 5.0×10<SUP>-5</SUP>-2.0×10<SUP>-4</SUP> M and slightly increased the number of discocytes at the higher concentration (2.0×10<SUP>-4</SUP> M). Therefore, progesterone has a direct and prompt effect on the erythrocyte membrane, and it seems to be inserted into the inner half of the membrane bilayer.

• 公益社団法人 日本薬理学会の論文

著者

• Saito Taiichi

  Department Of Neurosurgery Neurological Institute Tokyo Women's Medical University

• KAYA Harumi

  Department of Biochemistry Boston University School of Medicine

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