The measurement of platelet aggregation and ATP-release in mice with liver damage induced by carbon tetrachloride (CCl4) using a whole blood aggregometer.

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概要

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• Time course change in platelet function on liver damaged mice was studied by a whole blood aggregometer. The liver damaged animals were produced by single and multiple injections (p.o.) of 20% CCl<SUB>4</SUB> in olive oil to ddY mice (6 weeks). Platelet aggregation and ATP-release were induced with collagen (final conc. of 2 μg/ml), ADP (final conc. of 20 μM) and arachidonic acid (AA: final conc. of 100 μM). After a single injection of CCl<SUB>4</SUB>, platelet counts increased at 5 and 12 hr, and then they decreased from 24 to 120 hr. Multiple injections of CCl<SUB>4</SUB> resulted in a significant increase in platelet counts. A single injection of CCl<SUB>4</SUB> suppressed aggregation by collagen at 24 and 48 hr and diminished the rate of ATP-release from 12 to 48 hr. AA-induced platelet aggregation was depressed at 48 hr, and ATP-release was also diminished from 24 to 72 hr after a single injection. ADP-induced platelet aggregation was decreased 24 and 48 hr after a single injection and at 48 hr after multiple injections, while, the rate of ATP-release by ADP was significantly increased after single and multiple injections. These changes in platelet functions might be consistent with our original report on the alterations in coagulative and fibrinolytic activities with CCl<SUB>4</SUB>-induced liver injury.

• 公益社団法人 日本薬理学会の論文

著者

• OKAZAKI Masako

  Department of Pharmacology, Showa University School of Medicine

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• The measurement of platelet aggregation and ATP-release in mice with liver damage induced by carbon tetrachloride (CCl4) using a whole blood aggregometer.

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