Protease-antiproteaseの観点よりみた扁桃炎の研究 : 第1報 ヒト急性扁桃炎の血中線溶に関する研究
スポンサーリンク
概要
- 論文の詳細を見る
It has been shown that biological activities of protease and antiprotease have modified a various phase of some inflammations. In order to clarify the relationship between these enzymes and the process in tonsillitis, we accomplished a series of studies on the circulating blood and tonsillar tissues with tonsillitis from the aspect of prote ase-antiprote ase activity. At first, fibrinolytic activity in the blood with acute tonsillitis was observed, since various methods of measuring fibrinolytic activity have been recently developed. Affinity chromatography has been applied for estimating fibrinolytic activity, then we could measure accurately this enzyme activity in the blood. Furthermore, it became possible to determine fibrinogen or fibrin degradated products (FgDP or FDP) in the circulating blood. Prior to determination of fibrinolytic activity in the blood with acute tonsillitis, chemical properties and biological activi-ties of FgDP were studied in vitro. The method of producing FgDP in vitro was as follows. Urokinase solution was added to fibrinogen (bovine) solution rich in plasminogen, and then this mixture was incubated at 37-C for various minutes. From this fundamental experiment on FgDP, it was shown that clotting activity to thrombin was inhibited by early digested products (FgDP). On the observation of fibrinolytic activity in the blood with acute tonsillitis, fibrinogen was determined by the modified Quicks method. After plasminogen was separated from the plasma using affinity chromatography, it was activated to plasmin by streptokinase (SK). Plasmin (SK-activated) activity was determined by the plasminogen free fibrin plate(commercial). The level of FgDP or FDP was measured using the latex-aggregation method. In comparison with normal adults, fibrinogen content was significantly increased in the blood of patients with acute tonsillitis (p<0.001). Plasminogen content was significantly decreased (p<0.005) and the level of FgDP or FDP was significantly increased (p<0.01). From these results obtained, it is evident that fibrinolytic activity in the blood of the patients with acute tonsillitis is increased. Protease-antiprotease
- 社団法人 日本耳鼻咽喉科学会の論文
著者
関連論文
- Protease-antiproteaseの観点よりみた扁桃炎の研究 : 第4報 扁桃摘出術の後出血における血中線溶活性の意義
- Protease-antiproteaseの観点よりみた扁桃炎の研究 : 第2報 ヒト扁桃組織の線溶系関与物質に関する研究
- Protease-antiproteaseの観点よりみた扁桃炎の研究 : 第1報 ヒト急性扁桃炎の血中線溶に関する研究