ラット耳道腺腫瘍に関する研究 : 第1報耳道腺腫瘍初期病変とその酵素組織化学ならびに生化学的研究

概要

論文の詳細を見る
Wistar SLC rats were injected with 25mg/kg of 7, 12-Dimethylbenz (α) anthracene intravenously twice at 51 and 66 days of age, and also fed on a commercial diet containing 4-Dimethylaminostilbene, 0.004%, from the age of 57 days to the respective days of their decapitations. Either an atypical hyperplasia of the acinar cells or a microtumor was detected histologically in the Zymbals glands of almost all treated rats 9-11 weeks after the first injection. Macroscopical tumors were induced only in the ear duct regions of almost all treated animals more than 17 weeks after the treatment.Fresh cryostat sections of the Zymbals glands were serially examined by histochemical methods for SDH, LDH and G6PDH, 6, 11, and 17 weeks after the first injection. SDH and G6PDH activities decreased progressively in the tumorigenesis, but moderate LDH activity remained in the tumor tissues. Biochemical determinations for LDH and MDH in the Zymbals glands harvested serially 4-20 weeks after the first injection of DMBA indicated that the quotient of LDH/MDH began to elevate 8-11 weeks after the injection and elevated progressively in the tumorigenesis owing to a lower MDH.
社団法人日本耳鼻咽喉科学会の論文