各種肉芽の骨破壊に対する影響

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概要

• 論文の詳細を見る

• The precise role of skin and its products on chronic inflammatory bone destruction in middle ear cholesteatoma is still poorly understood.Inflammatory connective tissues produced in response to keratinizing epithelium, carrageenan and granuloma from open wound in the skull of rat were analyed for bone resorbing activity to determine their importance in chronic imflammatory bone resorption.Granuloma were induced in rats under the frontal scalp with three different methods:1. As the standard granuloma inducing agent, carrageenan in the form of 1% suspension was injected.2. Wounds were produced in the frontal area of the scalp by incising skin and suturing the edge back to prevent epithelial closure.3. Keratin and epithelium debris were removed from rat skin, dried, powered and injected as 1% suspension.The resulting granulation tissue was excised at one, two, and four weeks after induction, examined chemically.Prostaglandine E2 (PG E2) was measured by radioimmunoassay. Osteoclast activating factor (OAF) was determined with a pre-labeled fetal bone tissue culture assay. Leucine aminopeptidase (LAP) was determinend with a coloremetric assay.Although there was variability among different samples of each granuloma, keratin granuloma produced the highest OAF activity with little decline at four weeks after induction. OAF activity from carrageenan granuloma at one and two weeke is just slightly below the activity of keratin granuloma. Wound granuloma and keratin granuloma exhibited higher PG E2 levels than did carrageenan granuloma or normal skin. The highest levels of PG E2 were found two weeks after induction. Levels of LAP in various granulomas at one week after induction were considerably higher than that of normal skin, but differed little from one another.The presence of numerous macrophages in various granulomas correlated with the degree of activity of bone resorption noted chemically. This would tend to highlight the importance of OAF, PG E2 and LAP in the resorption induced by macrophage.It leads to the conclusion that keratin debris enhances the bone resorbing capacity of granulation tissue in rats just as it does in human otitis media.

著者

• 白幡 雄一

  東京慈恵会医科大学耳鼻咽喉科学教室

• アブラムソン マクスエル

  コロンビア大学医学部耳鼻咽喉科学教室

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