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The middle ear effusions (MEEs) by otitis media with effusion (OME) and acute otitis media (AOM) were analyzed for the content of proteins and three lysosomal enzymes; acid phosphatase, cathepsin D and arylsulfatase. According to the viscosity of MEEs, the MEEs by OME were subdivided into serous, seromucous and glue effusions. The results were follows.1) The content of Proteins was found to be higher in the MEEs than in the sera, but no significant difference was found among the MEEs. On the other hand, the content of proteins in the cell pellet in the MEEs was higher in glue MEEs than in serous MEEs.2) In all cases, specific activities of the three lysosomal enzymes were significantly higher in the MEEs than in the sera.3) Specific activities of these lysosomal enzymes in the serous MEEs were the lowest among MEEs of all groups.4) While the enzymatic activities in the supernatant of all MEEs were higher than in the pellet, no significant difference in the ratio of the supernatant to the pellet was found among MEEs of all groups.
一般社団法人日本耳鼻咽喉科学会の論文