A STUDY OF POSTMORTEM CHANGES OF THE SPIRAL ORGAN BY SCANNING ELECTRON MICROSCOPY
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概要
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Postmortem changes of the spiral organ in guinea pigs were observed on the scanmng electron microscope. The time-interval from death to fixation varied from zero to six hours. The results are as follows: Changes were observed both on the cell bodies and sensory hairs. There were bullae-formation and cytoplasmic protrusions from the cell surfaces of the cell bodies and fusion, distortion or loss of the sensory hairs. In the group kept at room temperature (19-25°C), bullae became apparent on the inner hair cells at ten minutes postmortem on the supporting cells of the outer hair cell region at one hour postmortem and on the outer hair cells at two hours postmortem. At four to six hours postmortem, destruction of surface structures were prominent accompanied with fusion, distortion or loss of sensory hairs. In the group kept in the refrigerator (4°C), the bullae-formation which was an initial sign of postmortem changes, became apparent at one hour postmortem on the hair cell regions and changes in the sensory hairs at four to six hours postmortem. The surface structures of the cell bodies were less severely affected than the sensory hairs in this group. In general, postmortem changes are more pronounced in the room temperature group than the refrigerator group. Morphological findings presented in this study can also be recognized in animals with various pathologies such as those induced by ototoxic drugs, noise trauma, blast injury and barotrauma. Therefore, it is important to be aware of these postmortem changes and to ascertain true pathologies when studying animal and human spiral organs by scanning electron microscopy.
- 一般社団法人 日本耳鼻咽喉科学会の論文