Histocytochemical SEM studies of taste cells utilizing both the secondary and back-scattered electron images.
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概要
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Freeze-cracked taste buds from rabbit foliate papillaes were studied using both the secondary electron (SE) image and the back-scattered electron (BSE) image. Zinc-Iodide-Osmium (ZIO) and Ruthenium red (RR) staining were used for SEM observation of the BSE image.With the SE image, the three-dimensional architecture of taste buds was relatively easy to visualize. However, individual cells could not be identified clearly. Basal cells were recognized easily from their position in the taste bud. The dense substance (DS) in the taste pore and the dark granules (DG) of the type I taste cell could be seen in the SE image. However, it was found that the BSE image produced by the ZIO method provided a strong contrast for both DS and DG that was superior to that of the conventional technique. Also a distinct BSE image could be obtained from the Golgi vesicles and DG near the Golgi apparatus at the perinuclear area in only the type I cell. It is of interest that DS and DG exhibit the same strong BSE image. It could be strongly suggested that the DG originated from the Golgi apparatus. RR, Which has an affinity for acid glycoconjugate, strongly stained the substance present along the membrane of cytoplasmic processes and some of the pore vesicles but DS and DG were only faintly stained or not at all in the BSE image. This implies that DS and DG contains only a small amount of polysaccharides. It appears then, that utilizing the BSE image in conjunction with the SE image is very useful in determinating the three-dimensional localization of histocytochemical components in taste buds.
- 一般社団法人 日本耳鼻咽喉科学会の論文