A study of Epstein-Barr virus susceptibility in human tonsil B lymphocyte subpopulations.
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概要
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Various subpopulations of human tonsil B lymphocyte were investigated for their susceptibility to infection by Epstein-Barr virus (EBV) by using monoclonal antibodies and flow cytometry. The EBV infection was detected by EBV binding and synthesis of EBV determined nuclear antigen (EBNA). All of the sorted resting B cells which reacted with L30, anti-IgM and-IgD antibodies synthesized EBNA 24hr after EBV infection. In contrast, the frequency of EBNA expression of OKT9-, L29- and OKT10-positive cells i.e., activated or differentiated B cells, and sIgG-positive cells tended to be low, as compared with those of marker-negative cells. However, the cells which were not reactive with OKB7 antibody, i.e., C3d receptor-negative B cells, were completely resistant to EBV infection. In the flow cytometric study using two-color immunofluorescence, EBV bound to all the cells which reacted with L30, anti-IgM, -IgD, -IgA, and-IgG antibodies, and on the most of OKT9- and OKT10-positive cells. In addition, EBV bidings, i.e., EBV receptors were present throughout the entire cell cycle. The study of density-fractionated B lymphocytes showed that the differences were not noticed in the frequency of the EBV binding on cells among the different fractions. However, high density fractionated cells could synthesize EBNA more frequently as compared with the cells in the low density fractions. In the analysis of B cells stimulated by the polyclonal activator in vitro, the expression of both EBV receptors and EBNA on these cells decreased and were finally lost at the terminal stage on the B cell lineage. The differences between EBV susceptibility in the various stages of the B cell lineage were discussed from these results.
- 一般社団法人 日本耳鼻咽喉科学会の論文