Immunohistochemical study of complements and complement receptor (CR1) in chronic sinusitis.
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概要
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Complements and complement receptor (CR1) in the maxillary mucosa were studied immunohistochemically in chronic sinusitis and in normal sinusoidal mucosa. The localization of mononuclear C3c, C1q, Factor B, C3b inactivator, and CR1 antibodies were investigated with peroxidaseantiperoxidase (PAP), avidinbiotin peroxidase complex (ABC), immunofluorescence methods, and with immunoelectron microscopy. Changes in the intensity of C3c fluorescence positivity was also investigated after the incubation of the specimens with exogene C3b.C3 was observed only in chronic sinusitis. It was localized on the surafce of the cilia, partially in the cytoplasm of the covering epithelium and in uneven distribution in the subepithelial stroma.CR1 was seen in the ciliar layer in both chronic sinusitis and normal maxillary mucosa. In chronic sinusitis C1q was not found and the localization of Factor B and C3b inactivator were similar to the one of C3 and CR1. Having treated with exogene C3b the intensity of fluorescence reaction was not increased in chronic sinusitis, but appeared in the normal mucosa.Our findings show that the CR1's function in chronic sinusitis was relatively decreased because of the CR1's binding capacity was exhausted by the combined C3b.The C1q negativity and the Factor B and C3b inactivator observed positivity in similar localization as C3 and CR1 was found suggest that the alternative pathway of complement activation plays a role in the generation of C3b.The maintenance of inflammation in chronic sinusitis is suspected to be caused by relative decrease of the CR1's function and mucociliary dysfunction brought about by complement components which degenerate the cilia and the epithelium.
- 一般社団法人 日本耳鼻咽喉科学会の論文