A study on endothelial cell growth factors from the thyroid anaplastic cell carcinoma using human umbilical vein-endothelial cells.
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Angiogenesis, the induction of formation of blood vessels, is critical to the development, proliferation and the metastasis of tumors. Several angiogenic factors, derived from human or mammalian tumors, have already been isolated or purified and will be closely related to both the therapeutic and diagnostic modalities of cancer.In the present study, the endothelial cell growth factors, isolated from the human thyroid anaplastic cell carcinoma, were carried on nude mice to partially characterize. The activities of growth promotion were monitored by 3H-thymidine incorporation DNA using cultured human umbilical vein endothelial cells.The crude extracts from the thyroid anaplastic tumors with 0.1M Tris-HCI, pH7.2, were subjected to fractionation with 35%, 50% and 100% ammonium sulfate, respectively. The precipitates of 35% to 50% showed prominent growth promotion activities and were further analyzed by Bio-Rex 70 cation exchange chromatography and heparin sepharose affinity chromatography. From the results of these chromatography, it was proved that these growth factors possesed cationic property and had affinity for heparin, thus showing differencies from normal tissue growth factors. The molecular weight determinations of these factors by HPLC gel chromatography showed approximately 41, 000 and 19, 000 daltons.These results suggested that the endothelial cell growth factors of thyroid anaplastic cell carcinoma tissues were mainly derived from the tumor cells. Analysis of these endothelial cell growth factors will be expected to establish methods for new laboratory examinations as well as new therapeutics in oncology.
- 一般社団法人 日本耳鼻咽喉科学会の論文