喉頭癌の原発巣と転移巣腫瘍細胞のレクチン組織化学
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In order to clarify possible alterations of membrane-, and cytoplasma-glycoconjugates of laryngeal cancer cells in metastatic process, a histochemical study was performed on laryngeal squamous carcinoma, using seven lectins conjugated with horseradish peroxidase (HRP); PNA, UEA-I, WGA, RCA-I, DBA, SBA and MPA. The author studied 32 primary tumors and 32 corresponding metastatic tumors obtained from 32 patients and primary tumors from 8 patientswithout histological evidence of lymph node metastasis. None of the patients underwent irradiation or chemotherapy before operation. The specimens were provided for routine lectin histochemistry. The present study revealed some significant differences in lectin-binding as follows.Primary tumor vs. metastatic tumor: There was a significant difference in lectin-binding between primary and metastatic cancer cells. 29 (90.0%) of 32 primary tumors were positive for MPA-staining. On the other hand, 21 (65.6%) of 32 metastatic tumors were positive for MPA-staining. There was a statistically significant (p<0.05) difference between primary and metastatic tumors with regard to MPA-binding. Primary tumor cells tended to more bind with lectins than with metastatic tumor cells.Well-differenciated primary tumor vs. moderately differenciated primary tumor: There was a significant difference in lectin-binding between these two types of tumors. Of 15 well-differenciated primary tumors, 13 (86.7%) showed SBA binding. The percentage of SBA-binding was significantly higher in well-differentiated tumor than in moderately differenciated primary tumors (50%, 8/16).Keratinization vs. non-keratinization: There was a significant difference in lectin-binding between keratinized and non-keratinized tumor cells in both primary and metastatic lesions. Keratinized metastatic tumor cells had significantly higher affinity to UEA-I, SBA and RCA-I than non-keratinized metastatic tumor cells. The percentages of SBA-, RCA-I- and UEA-I-stainings were 100%, 91.7% and 50%, respectively, for keratinized metastasis (n=12), and 70%, 30% and 5% for non-keratinized one (n=20).Primary tumor associated with cervical lymph node metastasis vs. primary tumor without cervical lymph node metastasis: 22 (68.8%) of 32 primary tumors associated with cervical lymph node involvement were positive for DBA-staining, whereas 8 primary tumors non-associated with this involvement were all negative. There was a significant difference between these two types of primary tumors with regard to DBA-staining. The above findings strongly suggest that glycoconjugates in cytoplasma and plasma membrane of cancer cells are altered in the process of metastasis, and closely related with metastatic potential and progression of laryngeal carcinoma.
- 一般社団法人 日本耳鼻咽喉科学会の論文