Tumor necrosis factor(TNF) production by peripheral monocytes in patients with inflammatory bowel disease(IBD).

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概要

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• We investigated tumor necrosis factor (TNF) production by peripheral monocytes obtained from 50 normal subjects and 28 patients with inflammatory bowel disease (IBD). Monocytes incubated with lipopolysaccharide (LPS) or phytohemagglutinin (PHA) for 20 h. TNF production and its release into the supernatant was analyzed by bioassay against L929 cells. Levels of TNF production by peripheral monocytes derived from normal subjects and patients with ulcerative colitis and Crohn's disease were 130±70U/ml, 1086±682 U/ml and 1035±682 U/ml, respectively, An elevated level of TNF production was thus demonstrated, which was thought to be due to increased LPS uptake. Positive rates of moncytes labelled with FITC-conjugated LPS were significantly higher in patients than in healthy controls. On the other hand, when the monocytes were treated with salazopirin and its metabolites (5-aminosalicylic acid and sulphapiridine) plus steroid, the level of TNF production was markedly suppressed. These results suggest an important role of TNF in the pathogenesis of IBD.

• 日本大腸肛門病学会の論文

著者

• 横山 昌樹

  弘前大学第2外科

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• Tumor necrosis factor(TNF) production by peripheral monocytes in patients with inflammatory bowel disease(IBD).

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