Histological and histochemical studies on colon carcinoma in rat induced by 1,2-Dimethylhydrazine.

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The colon of rat administrated with 1, 2-Dimethylhydrazine was studied histologically and histochemically.<BR>Sixteen weeks after administration of the carcinogen, adenomas were observed and after 19 weeks carcinomas, which showed tubular, signet-ring cell and mucinous carcinoma, were observed histologically.<BR>The histochemical localization of γ-glutamyl transpeptidase (GGT) and alkaline phosphatase (ALP) was investigated in these tumors. GGT activity was negative in normal colon mucosa. In adenoma, GGT activity was found in stroma especially under the surface epithelium. It was also highly positive in the stroma of carcinoma and localized on the carcinoma cells in some cases. ALP activity was positive only on the capillaries in normal colon mucosa. On the other hand ALP activity was positive diffusely in the stroma of adenomas and carcinomas. Ultrastructurally, ALP activity was noted on the plasma membrane of capillary endothelial cells, histiocytes, eosinophils and activated mesenchymal cells.<BR>It was suggested that the histological and histochemical studies on the carcinogenesis of rat colon were useful for elucidating the interaction between tumor cells and mesenchymal cells.
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