細胞情報伝達機構におけるCa<SUP>2+</SUP>/カルモジュリン依存性プロテインキナーゼIIの役割
スポンサーリンク
概要
- 論文の詳細を見る
Both Ca<SUP>2+</SUP>/calmodulin-dependent protein kinase II (CaM kinase II) and protein kinase C (PKC) have been implicated as possible candidates for contributing to the induction of long-term potentiation (LTP) in the hippocampus. The induction of LTP in the CA1 region of the hippocampus, an event which requires postsynaptic Ca<SUP>2+</SUP> influx through NMDA-type glutamate receptors, is blocked by calmodulin antagonists and inhibitors of CaM kinase II and PKC. In the present study, we describe the activation characteristics of CaM kinase II and PKC through the stimulation of glutamate receptors and regulation of the phosphorylation of substrates for CaM kinase II in the hippocampus. In cultured rat hippocampal neurons, glutamate elevated the Ca<SUP>2+</SUP>-independent activity of CaM kinase II through autophosphorylation, and this response was blocked by specific antagonists of the NMDA receptor. In addition, glutamate stimulated the translocation of PKC from the cytosol to the membrane fraction through the metabotropic glutamate receptor. In the experiments with <SUP>32</SUP>P-labeled cells, the phosphorylation of microtubule-associated protein 2 (MAP2) and synapsin I was stimulated by the exposure to glutamate. Finally, we demonstrated that high, but not low, frequency stimulation applied to two groups of CA1 afferents in the slices resulted in the induction of LTP with concomitant long-lasting increases in the Ca<SUP>2+</SUP>-independent and total CaM kinase II activities as well as the autophosphorylation. It could be blocked by preincubation of the slices with NMDA-receptor antagonist. These results suggest that glutamate can activate CaM kinase II through NMDA receptors in the induction of LTP and in turn stimulates the phosphorylation of target proteins such as MAP2 and synapsin I.
- 社団法人 日本薬理学会の論文
著者
関連論文
- 1-ブロモプロパン亜慢性曝露による海馬の過剰興奮と細胞内シグナル伝達マーカー
- プロリン指向性蛋白質燐酸化酵素によるMARCKSとMAP2の燐酸化反応
- Stimulation of cyclic adenosine 3´,5´- monophosphate-dependent proteinkinase with brain gangliosides
- 心肥大形成のカルシウムシグナル
- ラット視交叉上核におけるLTPの情報伝達系とメラトニンによる調節
- グルタミン酸受容体刺激に伴うCREBの燐酸化反応とメラトニンによる調節
- 1H1130 低濃度ホルムアルデヒド長期吸入曝露によるマウス海馬神経細胞の機能変化
- EDRF/NO合成酵素のCyclic AMP-dependent Protein Kinase(Aキナーゼ)による燐酸化及びその調節機構
- Ca^-カルモジュリン依存性プロテインキナーゼでリン酸化されたウシ脳ミエリン塩基性タンパク質のリン酸化部位(発表論文抄録(1987年))
- ウシ脳ミエリン塩基性タンパク中のリン酸化ベプチドの逆相高速液体クロマトグラフィーによる同定(発表論文抄録(1985年))
- 細胞情報伝達機構におけるCa2+/カルモジュリン依存性プロテインキナーゼIIの役割
- 細胞系におけるCa2+/カルモジュリン依存性プロテインキナーゼIIの細胞刺激に反応した調節 : シグナル伝達と細胞機能調節
- 第29回北米神経科学学会見聞記 : 神経可塑性の分子機構