Efficacy of amphotericin B liposomes bearing monoclonal antibody to Candida albicans.
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概要
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Liposomes expressing external antibody specific for <I>Candia albicans</I> and encapsulating amphotericin B were studied. Fluorescent microscopy indicated that the antibody showed specificity for the yeast cells of <I>C. albicans</I> and it was modified by papain to antibody fragments (Fab'). Fab' fragments were conjugated with egg phosphatidylcholine liposomes coated with pullulan derivatives bearing maleimide group and twenty-two Fab' fragments were connected with one liposome in this study. <I>Candida albicans</I> phagocytized by macrophage was mixed with free amphotericin B (FAMB), liposome-encapsulated amphotericin B (LAMB), and amphotericin B liposomes bearing antibody (LAMB-Ab), FAMB was the most effective but LAMB and LAMB-Ab showed similar effects against <I>C. albicans</I>. <I>Candida albicans</I> was mixed with FAMB or LAMB or LAMB-Ab and added to macrophages. FAMB showed the most efficient killing of the yeast cells and LAMB-Ab was more effective than LAMB but there was no significant difference between LAMB and LAMB-Ab. To investigate the efficacy of LAMB and LAMB-Ab in mice with disseminated candidiasis, the number of the fungi in lungs, liver, spleen and kidneys were examined. However there was no significant difference between LAMB and LAMB-Ab.
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