Cholesterol Effects on Endoglin and Its Downstream Pathways in ApoE/LDLR Double Knockout Mice
スポンサーリンク
概要
- 論文の詳細を見る
Background: The aim of the study was to evaluate whether cholesterol-rich diet affects transforming growth factor-β-RIII (endoglin) levels in blood and 2 endoglin-related pathways in the aorta of ApoE/LDLR double knockout mice. Methods and Results: Mice were fed either chow diet (CHOW) (n=8) or by 1% cholesterol-rich diet (CHOL) (n=8). Biochemical analysis of cholesterol and endoglin levels in blood, lesion size area, immunohistochemistry and Western blot analysis in mice aortas were performed. Biochemical analysis showed that cholesterol-rich diet resulted in a significant increase of cholesterol and endoglin levels in serum, and increased plaque size in the aorta. In addition, a cholesterol-rich diet significantly decreased the expressions of endoglin by 92%, activin receptor-like kinase (ALK)-1 by 71%, p-Smad2 by 21%, and vascular endothelial growth factor (VEGF) by 37% when compared to CHOW mice, but ALK-5, p-Smad1, and endothelial nitric oxide synthase were not significantly affected. Conclusions: Hypercholesterolemia increases endoglin levels in blood and simultaneously decreases its expression in aorta, together with atherosclerosis protective markers p-Smad2 and VEGF, followed by increased plaque size. Inhibition of endoglin signaling might be one of the mechanisms responsible for the promoting of endothelial dysfunction and atherogenesis. Moreover, the monitoring of endoglin serum levels might represent an attractive blood marker of progression of disease; however, the precise source and role of endoglin in blood serum remains to be elucidated. (Circ J 2011; 75: 1747-1755)
論文 | ランダム
- 日本近代史資料としての戦争記念碑--忠魂碑の史料論的考察 (特集 歴史を記す)
- 近代日本における戦争紀念碑と戦没者慰霊についての地域社会史的研究 (新年特集号 共同研究の成果とゆくえ) -- (共同研究の現在)
- 2002年度九州史学研究会大会公開講演 近代日本における戦争紀念碑と軍人墓(下)
- 近代日本における戦争紀念碑と軍人墓(上) (2002年度九州史学研究会大会公開講演)
- 書評 大濱徹也著『日本人と戦争』