Effects of Glucose and Amino Acids on In-Vitro Development of Rat Preimplantation Embryos, and Morphological Features of the Developed Blastocysts

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This study examined the effects of glucose and amino acids on in vitro development of Sprague-Dawley (SD) rat 1-cell embryos, and the morphological features of the developed blastocysts. Approximately 60% of one-cell embryos developed in vitro to the blastocyst stage when 0.5mM glucose was added to a chemically defined medium (modified hamster embryo culture medium-1; HECM-1), and glucose and amino acids had synergistic effects on the development. However, the mean cell number of the blastocysts developed in vitro from 1-cell stage embryos was significantly less compared with that of the blastocysts developed in vivo (P<0.01). The morphological features of 1-cell embryos developed in vitro to the blastocyst stage differed somewhat to those of the in vivo-developed blastocysts; less development of blastocoele, narrow intercellular space in inner cell mass and high electron-dense mitochondria. These results suggest that SD rat 1-cell embryos can developed in vitro to the blastocysts stage, and glucose and amino acids have important roles in the embryonic development. However, the blastocysts developed in vitro from 1-cell embryos may have some differences in comparison with the blastocysts developed in vivo.
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