Genetic Analysis of Japanese Sika Deer in Nikko National Park by Random Amplified Polymorphic DNA Method
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概要
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Genetic variation in Japanese sika deer populations in Nikko National Park including Omote-Nikko, Ashio and Oku-Nikko areas in Japan was examined by random amplified polymorphic DNA method (RAPD). DNA samples extracted from fresh blood were amplified by polymerase chain reaction (PCR) using five kinds of primers. RAPD patterns were determined according to the site and the number of bands displayed by electrophoresis. Polymorphism in RAPD patterns with three primers was found in PCR products obtained from Nikko deer. The results in Nikko deer were compared with RAPD patterns of DNA samples of deer in Tsushima island. Based on the number of bands in RAPD patterns, band-sharing coefficients (BS) within Nikko and Tsushima populations were calculated, and genetic variation in Nikko population was higher than that in Tsushima population. Homogeneity between populations was the highest between Omote-Nikko and Ashio populations among Omote-Nikko, Ashio and Oku-Nikko populations. We concluded that Oku-Nikko population had been derived from Omote-Nikko and Ashio populations after 1984, and the number of deer in this area increased rapidly in a short time.
- 公益社団法人 日本畜産学会の論文
公益社団法人 日本畜産学会 | 論文
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