副腎皮質機能の反芻動物栄養における特異性に関する研究-1-血漿中および副腎中の副腎皮質ホルモンの微量定量法,ならびにコルチゾールとコルチコステロンとの分画法についての検討
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A method for the microestimation of adrenocorticoids and for the fractionation of both cortisol and corticosterone was investigated in plasma and adrenal tissues of cattle, sheep, rabbits and rats.1) The determination of 11 OHCS in plasma and adrenal tissues.The method for the determination of 11 OHCS in plasma is almost identical with that of Braunsberg (1962). In addition, improvements were made on a few points. The recoveries of cortisol and corticosterone added to plasma were 97.4±9.0% and 101.2±10.4%, respectively.Various methods of measurement for 11 OHCS extracted from plasma were compared and discussed. As the result, the fluorometric method, using ethanolic-sulphuric acid, was excellent in regard to the sensitivity and specificity.A method which can be applied to the extraction of both cortisol and corticosterone in adrenal tissues was established. The recoveries of cortisol and corticosterone added to adrenal tissue homogenates were 85.3±11.1% and 108.0±12.0%, respectively.2) On the fractionation of cortisol and corticosterone.The 11 OHCS was separated into cortisol and corticosterone by Thin Layer Chromatography (TLC), which Kieselgel H was used as adsorbent. The mixed solvent of chloroform and methylene chloride (90:10v/v) was used as the developer. The elution apparatus with glass filter, was made for the isolation of cortisol and corticosterone from Kieselgel H. The recoveries of cortisol and corticosterone added on TLC were 81.0±9.7% and 81.5±13.8%, respectively.3) Some of the examples were analyzed by the method established in this experiment. From the results obtained here, the authors suppose that the analytical method mentioned in this paper should be adequate for the analysis of adrenocorticoids, especially 11 OHCS.
- 公益社団法人 日本畜産学会の論文
公益社団法人 日本畜産学会 | 論文
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