クロムとDNP-アミノ酸との反応におよぼす基礎的因子の影響(クロム塩とα-カルボキシル基との反応に関する研究-1-)

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The reaction of Cr salt with DNP-amino acid was studied under various conditions. Sulfur dioxide-reduced liquors containing DNP-glycine were used. The amount of DNP-glycine combined with Cr, which was not removed with ether, was determined from the difference in the amounts of uncombined DNP-glycine. The amount of uncombined DNP-glycine was calculated from the difference in absorbancies which had been measured at a wave length of 350 mμ before an after extracton with ether. Then the effects of concentration of Cr, time of reaction, pH and addition of some organic salts upon the amount of reaction of Cr with α-carboxyl group were studied in comparison with relative values obtained for the combined DNP-glycine. The results obtained are summerized as follows: 1. The amount of combined DNP-glycine increased as the concentration of Cr became higher. 2. The reaction between them proceeded fast until the standing of the solution lasted for 72 hours and then it slowed down later. 3. The amount of combined DNP-glycine increased with rising of pH to a maximum value at nearly original pH 4 and then decreased. 4. The amount of combined DNP-glycine decreased by addition of Na-acetate and -oxalate.
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