家兎の誘起排卵に及ぼす活性炭処理豚卵胞液の影響〔英文〕

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Effects of charcoal-treated porcine follicular fluid (CTPFF) on ovulation in rabbits were examined. Sixty-one does were divided into 4 groups. Group 1 showed that an intravenous injection of 15IU human chorionic gonadotropin (hCG) gave rise to ovulation in all 4 does either treated with CTPFF or control. Does in group 2 received 4ml of whole PFF (WPFF) or CTPFF once daily for 10 days, followed by 5IU hCG. With WPFF administration, 1 out of 4 does ovulated, while 3 out of 4 does ovulated in the CTPFF and control groups. A mild aplasia of corpora lutea was noted in does which received CTPFF. In group 3, 10 administrations of CTPFF were made at successive 12-hour intervals followed by 10IU hCG at the final injection of CTPFF; 3 out of 6 does ovulated (6 out of 8 does in control group). The number of ovulations was significantly lower in the CTPFF group than in controls. Laparotomy 4 days after hCG treatment showed that the number of large follicles was smaller in ovaries of nonovulated does in the treated group than in controls. Size and coloration of vulvas decreased 2 days after the beginning of treatments. In group 4, effects of CTPFF administration 3 times and 2 times per day were compared. Each doe was mated only 3 times of copulations without hCG. No ovulations occurred in both CTPFF-treated groups, though does ovulated were 2 out of 10 in 3 times saline-treated controls and 2 out of 6 in 2 times saline-treated control groups. Macroscopic measurements of follicles in non-ovulated does, and appearance of vulvar swelling and coloration showed similar trends with group 3. These result suggest that follicle-stimulating hormone suppressive activities in CTPFF will suppress follicular development in rabbit ovaries (relating low ovulation rate) and this causes a decrease of estrogen levels in blood, leading the fading and/or withering of vulvas.
社団法人日本畜産学会の論文

家兎の誘起排卵に及ぼす活性炭処理豚卵胞液の影響〔英文〕

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