NMRから見たpolypeptideのhelix-coil転移の速度

概要

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A number of experiments have been performed on the kinetics of the helix-coil transition of polypeptides and the reported results are devided into two classes: "fast" and "slow". Schwarz calculated the kinetic characteristics of the transition taking into account of the nucleation and propagation process in the formation of the helical structure and arrived at the expectation of the relaxation time which time which falls in submicro second range even at the transition midpoint. Experiments performed by temperature jump techniques, dielectric dispersion and ultrasonic methods have supported above expectation and all of them measure times of the order of 10-7sec. On the other hand relaxation times, as measured by NMR, are reported to be greater than 10-2sec which are derived from the analysis of the double peak in NMR spetra seen near the transition midpoint. What is the reasen of this discrepancy between fast and slow relaxation times? We review this problew focussing our attention to the NMR experiments and examine the background of this contradiction.
日本生物物理学会の論文