Keratinocyte Growth Factor (KGF) Gene Therapy Mediated by an Attenuated Form of Salmonella typhimurium Ameliorates Radiation Induced Pulmonary Injury in Rats
スポンサーリンク
概要
- 論文の詳細を見る
The aim of this study is to investigate the effect of KGF (Keratinocyte growth factor) gene therapy mediated by the attenuated Salmonella typhimurium Ty21a on radiation-induced pulmonary injury in rats model. Sprague–Dawley rats were divided into three groups: TPK group (treated with TPK strain, attenuated Salmonella typhimurium Ty21a-recombined human KGF gene); TP group (treated with TP strain, attenuated Salmonella typhimurium Ty21a-recombined blank plasmid); and Saline group (treated with saline). After intraperitoneal administration for 48 h, the thoraxes of the rats were exposed to X-ray (20 Gy), and the rats were administered again two weeks after radiation. On the 3rd, 5th, 7th, 14th and 28th day after radiation, the rats were sacrificed and lung tissues were harvested. Histological analysis was performed, MDA contents and SOD activity were detected, mRNA levels of KGF, TGF-β, SP-A and SP-C were measured by Real-time RT-PCR, and their concentrations in the BALF were quantified with ELISA. Administration of TPK strain improved the pathological changes of the lung on the 28th day. In the TPK group, KGF effectively expressed since the 3rd day, MDA contents decreased and SOD activity increased significantly, on the 7th day and 14th day respectively. SP-A and SP-C expression elevated, whereas TGF-β expression was inhibited in the TPK group. These results suggest that this novel gene therapy of KGF could ameliorate radiation-induced pulmonary injury in rats, and may be a promising therapy for the treatment of radiative pulmonary injury.
論文 | ランダム
- Anti-Inflammatory Effects of Seeds of the Tropical Fruit Camu-Camu (Myrciaria dubia)
- 相互作用と適応化を考慮したクラスタ構造型Particle Swarm Optimization
- 増殖糖尿病網膜症に伴う虹彩隅角新生血管に対する治療予後
- 129)僧帽弁狭窄症における造影CTによる左房内血栓の検討 : 日本循環器学会第65回東海・第50回北陸合同地方会
- 44)運動負荷心筋シンチグラフィの定量評価法の検討 : 日本循環器学会第56回東海・第41回北陸合同地方会